Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets
in Skeletal Muscle
Figure 3
Staining of LDs with BODIPY (493/503) in cross section of rat tibialis anterior (TA) muscle demonstrates that LDs are not limited to muscle fibers expressing slow MHC. (a) Fibers stained with BODIPY (green) demonstrate the appearance of spherical LDs within the muscle fibers as previously described with Oil Red O staining procedures. (b) Slow fibers were labeled using an antibody specific for the slow form of MHC (red). (c) Nuclei were stained with DAPI (Blue). (d) Merged triple label image of the muscle section demonstrates fibers presenting with substantial BODIPY signal that do not label for slow MHC. All images were taken at 10x, and a scale bar is provided for reference.
