Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets
in Skeletal Muscle
Figure 5
Exposure of single fibers to a palmitate-oleate mixture substantially increases the size and frequency of LDs within cultured mouse single muscle fibers. (a, c) Single muscle fibers isolated from the FDB were doubly stained with BODIPY (493/503-green) and DAPI (blue). The fibers were cultured overnight in standard media conditions. A heterogeneous mix of fibers presenting with LDs was found, in which some fibers presented with a visible number of LDs (a), while others presented with virtually no LDs (c). (b, d) Single muscle fibers isolated from the FDB were cultured overnight in standard media conditions supplemented with albumin-conjugated palmitate-oleate mixture (0.5 mM : 0.5 mM ratio). A substantially higher number and larger LDs were found consistently across all fibers that were imaged. All images were taken at 63x, and a scale bar is provided for reference.
