The Role of Synthetic Biology in the Design of Microbial Cell Factories for Biofuel Production

Figure 5

Metabolically engineered Escherichia coli strain for microbial production of free fatty acids. Deleted or attenuated genes are indicated by crosses. Gene/enzyme names: fadE (genes encoding for β-oxidation pathway); tesA (thioesterase); fadD (acyl-CoA synthase); accABCD (acetyl-CoA carboxylase); fabH, fabD, fabG, fabF (fatty acid synthase); aceEF (pyruvate dehydrogenase and pyruvate formate lyase); gpsA (glycerol-3-phosphate dehydrogenase); plsB (glycerol-3-phosphate O-acyltransferase); pta (phosphate acyltransferase); ackA (acetate kinase).