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617974.fig.005b
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Figure 5: APP fragments were detected in mouse brains by Western blot analyses. Ten or twenty micrograms of whole brain proteins were prepared from each genotype at 10-11 months of age. (a) Rodent and human APP full-length (APP-FL) and (b) C-terminal fragments (β- and α-CTF) were detected with the anti-APP C-terminus specific antibody. (c) The α-cleavage soluble APP (sAPPα) band was observed with anti-sAPPα C-terminus specific antibody. (d) The β-cleavage soluble APP (sAPPβ) band appeared with anti-sAPPβ C-terminus specific antibody. (e) Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal standard.