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Figure 3: Micrographies of ML parasites cultures in presence of sexual steroids. Parasites cultured at 36 hr with progesterone (P4, 100 mM), estradiol (E2, 50 nM), or testosterone (T4, 50 nM) or in absence of sex steroid (control) were observed in Axiovert microscopy using 25x objective. In all cases, the old cuticle starts to detach (clear zone) at the apical or basal of the parasite (arrows).