Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis

Figure 3

Specific peptides obtained by MALDI TOF/TOF (trypsin, Asp-N, and Lys-C proteases) and highlighted in multiple sequence alignment (ClustalW 1.83) of MyHCα and MyHCβ: yellow: HY MyHCβ, blue: EU MyHCα, green: TH MyHCα, pink: overlap of EU MyHCα and TH MyHCα. Red letters: specific amino acids for each MyHC isoform.