Myosin Binding Protein-C: A Regulator of Actomyosin Interaction in Striated Muscle

Table 1

Ligands of the cardiac isoform of MyBP-C.

Cardiac MyBP-C
MyBP-C Domain	Ligand	Method	Affinity	Reference
C0	Myosin RLC	NMR, ITC	3.2 μM	[32]
C1	Myosin S1-S2 hinge region	NMR		[33]
M-motif	Myosin S2	Cosedimentation assay, ITC	4.3 μM	[34]
C2	Myosin S2	ITC, NMR	1.1 mM	[35]
C10	Myosin LMM	Cosedimentation	3.5 μM	[28, 29, 36]
C0	F-actin	Co-IP, cosedimentation		[37]
C0 and C1	F-actin	Small angle neutron scattering		[38]
C0–C2	F-actin	Negative staining electron microscopy		[39]
C1-C2	F-actin	Cosedimentation	10 μM	[40]
M-motif	F-actin	Cosedimentation		[41]
C5–C10	F-actin	Cosedimentation	4.3 μM	[42]
C5	MyBP-C C8	Y2H, SPR	10 μM	[43]
C7	MyBP-C C10	Y2H		[43]
C8–C10	Titin C-Zone 11-repeat super repeat	Dot-blot		[31]

Abbreviations used: RLC, regulatory light chain; NMR, nuclear magnetic resonance; ITC, isothermal calorimetry; LMM, light meromyosin; Co-IP, coimmunoprecipitation; Y2H, yeast 2 hybrid; SPR, surface plasmon resonance.