Myosin Binding Protein-C: A Regulator of Actomyosin Interaction in Striated Muscle

Table 2

Ligands of the skeletal isoforms of MyBP-C.

Skeletal MyBP-C
MyBP-C Domain	Ligand	Method	Affinity	Reference
C1-C2	Myosin S2	Cosedimentation assay, ITC	2.2 μM	[26, 34]
fast C10	Myosin filaments	Cosedimentation assay	0.5 μM	[27]
Full length	F-actin	Cosedimentation		[36, 50]
Full length	Titin 11-domain super repeat	Dot-blot		[31, 54]
slow variant 1 C10 + insert	Obscurin Ig2	Y2H, pull down, overlay		[13]
slow variant 1 C10 + insert	FHL1	Y2H, pull down, Co-IP		[55]

Abbreviations used: RLC, regulatory light chain; NMR, nuclear magnetic resonance; ITC, isothermal calorimetry; LMM, light meromyosin; Co-IP, coimmunoprecipitation; Y2H, yeast 2 hybrid; SPR, surface plasmon resonance.