Investigation of a Potential Scintigraphic Tracer for Imaging Apoptosis: Radioiodinated Annexin V-Kunitz Protease Inhibitor Fusion Protein

Figure 3

Representative flow cytometric histograms of apoptotic Jurkat T-cells stained with annexin V-FITC and propidium iodide (PI). Jurkat T-cells (2×10⁶/mL) were left untreated or treated with camptothecin (CPT, 8 μM) at 37°C for 24 h, followed by staining with ANV-FITC and PI. Apoptotic cells were identified as ANV⁺PI^- and live cells as ANV^-PI^- on an FACScan analyser.

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