Antioxidant and Antinociceptive Effects of Citrus limon Essential Oil in Mice

Table 1

Effects of essential oil (EO) of C. limon, morphine (MOR), and naloxone (NAL) on writhing induced by acetic acid.

Treatment	Dose (mg/kg)	No. of writhings*	% inhibition
Vehicle	—	14.6 ± 2.7	—
EO	50	3.7 ± 0.6^a	75
EO	100	3.6 ± 0.82^a	76
EO	150	2.9 ± 0.9^a,b,c	81
EO plus NAL	150 + 1.5	10.5 ± 1.7^a,d	27
MOR	5	1.6 ± 0.5^a	91
MOR plus NAL	5 + 1.5	11.2 ± 1.9^e,f	23

𝑛=7 per group.
*Values represent mean ± SEM.
^a𝑃<.01, significantly different from control, ^b𝑃<.01, significantly different from EO 50 group, ^c𝑃<.01, significantly different from EO 100 group. ^d𝑃<.01, significantly different from EO 150 group, ^e𝑃<.01, significantly different from EO plus NAL group, ^f𝑃<.01, significantly different from MOR group. One-way ANOVA followed by Dunnett’s test was applied to all data.