Antioxidant and Antinociceptive Effects of Citrus limon Essential Oil in Mice

Table 2

Effect of essential oil (EO) of Citrus limon, morphine (MOR) and aspirin on formalin-induced pain.

Treatment	Dose (mg/kg)	No. of licks (s)
		0–5 min		15–30 min
		Score of pain*	% inhibition	Score of pain*	% inhibition
Vehicle	—	62.06.2±	—	64.0±11.2	—
EO	50	55.1±6.6^a	11	63.4±9.1	11
EO	100	39.9±9.8^a,b	36	9.1±4.4^a,b	85
EO	150	11.8±1.9^a,b,c	81	5.2±0.4^a,b,c	92
MOR	5	2.95±0.9^a	95	3.89±0.1^a	94
Aspirin	200	45.4±9.8^a,d	27	4.9±0.6^a,d	92

𝑛=7 per group.
*Values represent mean ± SEM.
^a𝑃<.01, significantly different from control, ^b𝑃<.01, significantly different from EO 50 group, ^c𝑃<.01, significantly different from EO 100 group, ^d𝑃<.001, significantly different from MOR group. One-way ANOVA followed by Dunnett’s test was applied to all data.