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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 691493, 7 pages
Methodology Report

Employment of Oligodeoxynucleotide plus Interleukin-2 Improves Cytogenetic Analysis in Splenic Marginal Zone Lymphoma

1Section of Haematology, Department of Bio-Medical Sciences and Advanced Therapies, University of Ferrara, Ferrara, Italy
2Department of Onco-Hematology, IRCCS, Centro di Riferimento Oncologico della Basilicata, Via Padre Pio 1, 85028 Rionero in Vulture (Pz), Italy

Received 16 October 2010; Revised 21 February 2011; Accepted 15 March 2011

Academic Editor: Anita M. Oberbauer

Copyright © 2011 Antonella Bardi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To compare the efficiency of novel mitogenic agents and traditional mitosis inductors, 18 patients with splenic marginal zone lymphoma (SMZL) were studied. Three cultures using oligodeoxynucleotide (ODN) plus interleukin-2 (IL-2), or TPA, or LPS were setup in each patient. Seventeen/18 cases with O D N + I L 2 had moderate/good proliferation ( 9 4 , 4 % ) as compared with 10/18 cases with TPA and LPS ( 5 5 % ) ( 𝑃 = . 0 1 5 ); 14/18 ( 7 7 , 7 % ) cases with O D N + I L 2 had sufficient good quality of banding as compared with 8/18 cases ( 4 4 , 4 % ) with TPA and LPS. The karyotype could be defined from O D N + I L 2 -stimulated cultures in all 18 patients, 14 of whom ( 7 7 , 7 % ) had a cytogenetic aberration, whereas clonal aberrations could be documented in 9 and in 3 cases by stimulation with LPS and TPA, respectively. Recurrent chromosome aberrations in our series were represented by aberrations of chromosome 14q in 5 patients, by trisomy 12 and 7q deletion in 4 cases each, and by abnormalities involving 11q and 13q in two cases each. These findings show that stimulation with O D N + I L 2 offers more mitotic figures of better quality and results in an increased rate of clonal aberrations in SMZL, making this method ideal for prospective studies aiming at the definition of the prognostic impact of cytogenetic aberrations in this disorder.