Production of a Solvent, Detergent, and Thermotolerant Lipase by a Newly Isolated Acinetobacter sp. in Submerged and Solid-State Fermentations

Table 1

Experimental design used in ANN studies by using five independent variables showing observed values of lipase activity in SmF.

Run	Temperature (°C)	pH	Inoculum (%)	Time (h)	Agitation (rpm)	Lipase activity (U/mg protein)
1	40.9	8.3	1.9	79.8	45.1	8.0
2	40.9	6.7	4.1	79.8	45.1	3.6
3	31.1	8.3	4.1	40.2	154.9	10.6
4	40.9	8.3	4.1	40.2	45.1	5.5
5	40.9	8.3	1.9	40.2	154.9	4.8
6	40.9	6.7	1.9	79.8	154.9	2.4
7	31.1	6.7	4.1	79.8	154.9	7.2
8	31.1	8.3	1.9	79.8	154.9	8.5
9	40.9	6.7	4.1	40.2	154.9	5.6
10	31.1	8.3	4.1	79.8	45.1	5.2
11	31.1	6.7	1.9	40.2	45.1	8.3
12	27.0	7.5	3.0	60.0	100.0	11.2
13	45.0	7.5	3.0	60.0	100.0	9.4
14	36.0	6.0	3.0	60.0	100.0	9.8
15	36.0	9.0	3.0	60.0	100.0	15.0
16	36.0	7.5	1.0	60.0	100.0	9.2
17	36.0	7.5	5.0	60.0	100.0	7.6
18	36.0	7.5	3.0	24.0	100.0	9.2
19	36.0	7.5	3.0	96.0	100.0	8.4
20	36.0	7.5	3.0	60.0	0	4.2
21	36.0	7.5	3.0	60.0	200.0	10.6
22	36.0	7.5	3.0	60.0	100.0	7.2
23	36.0	7.5	3.0	60.0	100.0	7.6
24	36.0	7.5	3.0	60.0	100.0	5.9
25	36.0	7.5	3.0	60.0	100.0	8.1
26	36.0	7.5	3.0	60.0	100.0	8.6