Proteomic Analysis of Sera from Common Variable Immunodeficiency Patients Undergoing Replacement Intravenous Immunoglobulin Therapy

Table 3

Relative expression and MALDI-TOF-MS-based identification of deregulated spots, as revealed by 2D-DIGE analysis of serum samples.

	Naive/Normal donors		Naive/IVIg donors		IVIg/Normal donors		Protein identification
Spot; no.	Av. Ratio	t-test	Av. Ratio	t-test	Av. Ratio	t-test	Protein name	Gene name	Accession number	Matched peptides	Sequence coverage	Est’d Z score	MW (theor)	pI (theor)
410	4.51	0.013	1.35	0.30	3.33	0.0018
416	2.02	0.21	1.25	0.67	1.62	0.031	Alpha-1-antitrypsin	SERPINA1	P01009	10	28%	2.34	44324	5.37
417	1.89	0.047	1.21	0.33	1.56	0.0081
518	4.92	0.12	1.31	0.91	3.77	0.0051
517	5.49	0.086	1.41	0.74	3.89	0.013	Alpha-2-macroglobulin	A2M	P01023	17	17%	2.32	160796	5.95
521	5.94	0.087	1.49	0.71	4.00	0.0082
550	2.20	0.010	1.10	0.58	1.99	0.042
557	2.47	0.0072	1.02	0.83	2.42	0.053	Ceruloplasmin	CP	P00450	12	25%	2.34	120085	5.41
558	2.52	0.0027	−1.00	0.90	2.53	0.042
564	7.48	0.11	1.04	0.78	7.16	0.013	Alpha-2-macroglobulin	A2M	P01023	9	8%	1.96	160796	5.95
623	4.27	0.048	1.07	0.66	3.99	0.29	Alpha-2-macroglobulin	A2M	P01023	12	10%	2.33	160796	5.95
666	7.08	0.0047	1.05	0.72	6.72	0.036
668	2.75	0.043	1.21	0.57	2.27	0.035
670	5.72	0.010	1.23	0.55	4.63	0.023	Complement factor B	CFB	P00751	20	32%	2.38	83000	6.66
680	6.20	0.013	1.32	0.50	4.71	0.046
709	5.22	0.065	2.79	0.10	1.87	0.014
795	−1.96	0.022	−1.42	0.061	−1.38	0.033	Afamin	AFM	P43652	8	13%	2.25	66577	5.58
1673	2.61	0.12	−1.41	0.44	3.68	0.039			Not identified
1772	2.53	0.016	1.30	0.17	1.94	0.025	Haptoglobin	HP	P00738	12	32%	2.35	43349	6.13
1811	−2.32	0.031	−1.55	0.19	−1.50	0.19	Ficolin-3	FCN3	O75636	7	19%	1.95	30353	6.22
1812	−2.52	0.043	−1.42	0.27	−1.78	0.084
1831	−2.75	0.047	−1.53	0.22	−1.79	0.091	Clusterin	CLU	P10909	6	11%	1.89	50062	5.89
1844	−2.54	0.047	−1.32	0.40	−1.91	0.081
1950	−2.41	0.045	−1.87	0.15	−1.29	0.41			Not identified
2288	−4.62	0.0042	−1.72	0.19	−2.68	0.053	Apolipoprotein A-I	APOA1	P02647	11	31%	2.24	28078	5.27
2334	−4.99	0.012	−1.83	0.41	−2.73	0.14	Apolipoprotein A-I	APOA1	P02647	14	43%	2.36	28078	5.27
2605	−3.46	0.013	−1.22	0.43	−2.84	0.011			Not identified