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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 707985, 11 pages
http://dx.doi.org/10.1155/2011/707985
Research Article

Experimental Model of Zymosan-Induced Arthritis in the Rat Temporomandibular Joint: Role of Nitric Oxide and Neutrophils

1Faculty of Dentistry, Federal University of Ceará, Sobral Campus, Avenida Comandante Maurocélio Rocha Pontes, 100 Derby, 62042-280 Sobral, CE, Brazil
2Faculty of Medicine of Sobral , Federal University of Ceará, Sobral Campus, Avenida Comandante Maurocélio Rocha Pontes, 100 Derby, 62042-280 Sobral, CE, Brazil
3Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Rua Coronel Nunes Valente, 1315 Rodolfo Teófilo, 60430-270 Fortaleza, CE, Brazil
4Department of Morphology, Faculty of Medicine, Federal University of Ceará, Rua Delmiro de Farias, Porangabussu, 60440-261 Fortaleza, CE, Brazil

Received 15 September 2010; Revised 2 December 2010; Accepted 9 December 2010

Academic Editor: Oreste Gualillo

Copyright © 2011 Hellíada Vasconcelos Chaves et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. To establish a new model of zymosan-induced temporomandibular joint (TMJ) arthritis in the rat and to investigate the role of nitric oxide. Methods. Inflammation was induced by an intra-articular injection of zymosan into the left TMJ. Mechanical hypernociception, cell influx, vascular permeability, myeloperoxidase activity, nitrite levels, and histological changes were measured in TMJ lavages or tissues at selected time points. These parameters were also evaluated after treatment with the nitric oxide synthase (NOS) inhibitors L-NAME or 1400 W. Results. Zymosan-induced TMJ arthritis caused a time-dependent leucocyte migration, plasma extravasation, mechanical hypernociception, and neutrophil accumulation between 4 and 24 h. TMJ immunohistochemical analyses showed increased inducible NOS expression. Treatment with L-NAME or 1400 W inhibited these parameters. Conclusion. Zymosan-induced TMJ arthritis is a reproducible model that may be used to assess both the mechanisms underlying TMJ inflammation and the potential tools for therapies. Nitric oxide may participate in the inflammatory temporomandibular dysfunction mechanisms.