Figure 2: Temporal profile (0–48 h) of the inflammatory and hypernociceptive effects of zymosan-induced TMJ arthritis. Zymosan (2 mg; 40 μL) or saline was injected i.art. into the left TMJ of the rat. The mechanical nociceptive threshold was measured before and after an i.art. injection of zymosan or saline until the 48th hour (a). Leukocyte migration was evaluated by cell counting in TMJ fluid (b). MPO activity from TMJ fluid (c) and TMJ tissues (d). Plasma extravasation (PE) following an i.art. injection of zymosan or saline (e). Data are expressed as means ± SEM (). versus Sham (ANOVA, Bonferroni).