Research Article

Novel Stromal Biomarkers in Human Breast Cancer Tissues Provide Evidence for the More Malignant Phenotype of Estrogen Receptor-Negative Tumors

Figure 1

2DE: two-dimensional gel electrophoresis (2DE) of epithelial cells (Epi), myofibroblasts (Myo), leukocytes (leuk), and endothelial cells (Endo) proteins separated from estrogen receptor-positive and estrogen receptor-negative breast cancer cells using antibody-bound magnetic beads. First dimension was performed on a pH 4 to 7 isoelectric focusing gels and the second dimension was done utilizing 10% polyacrylamide gels. Gels were stained by Sypro Ruby. Arrows point to identified proteins spots.
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