Antioxidant activity (%) of algal crude extract, spring-collected ethyl acetate (EA) extract and standard BHT by different antioxidant methods at 100 and 200 μg/mL.
Extract
DPPH
ABTS
Total antioxidant
Reducing power
β-carotene bleaching
100
200
100
200
100
200
100
200
100
200
Crude (ethanolic extract)
46 ± 1.6
55.7 ± 1.9
30.3 ± 0.90
34.5 ± 0.7
2.7 ± 0.3
9.0 ± 0.2
5.15 ± 1.0
6.18 ± 1.0
65 ± 1.0
67 ± 1.88
EA (spring)
79.5 ± 4.6
95.7 ± 7.9
16.5 ± 0.6
20.5 ± 1.2
10.7 ± 0.9
17.50 ± 0.1
6.5 ± 0.3
7.47 ± 0.5
18 ± 1.2
20 ± 2.6
BHT
83 ± 2.8
88 ± 6.3
83.6 ± 4.9
84.6 ± 5.9
32.20 ± 1.6
37.1 ± 4.6
15.03 ± 2.3
18.12 ± 2.1
66 ± 3.7
68 ± 1.8
LSD
0.338
0.338
0.041
0.041
4.130
4.130
0.024
0.024
0.413
0.413
Each value is presented as mean of triplet treatments; LSD: Least significant difference at P ≤ 0.01 according to Duncan’s multiple range test.
