Synergistic Antitumor Effect of Dichloroacetate in Combination with 5-Fluorouracil in Colorectal Cancer

Table 2

SW620, LoVo, LS174t, HT29 cells, and 293T noncancerous controls were treated with 10 mM DCA and 20 μM 5-FU alone or in combination for 48 hours, and then pulsed with BrdU. Cells were then harvested and stained, and the numbers of BrdU+ cells were calculated as the mean number of the positive cells in eight different fields of view in one image (magnification, 400X). This calculation was repeated three times. *𝑃<.05, compared with control. BrdU⁺  cells in different drug treatment (%, mean ± SD).

Drugs	BrdU^+  rate
	SW620	LoVo	LS174t	HT29
untreated	39.70±2.13	40.54±1.28	38.53±0.94	42.14±3.18
5-FU	25.2±2.40	27.33±1.47	27.94±0.83	40.53±2.71
DCA	30.4±1.25	28.25±2.17	31.72±0.73	29.94±2.16
5-FU+DCA	11.4±2.12	13.55±3.10	12.84±1.34	18.83±1.45