Table 1: Primers used for real-time PCR.

GeneForward PrimerReverse Primer

ANF5′-CAGGGCAAACAGGAGCAAA-3′5′-TCCATCAGGTCTGCATTGGA-3′
CTGF5′-TCCCACGGAGGGTCAAACT-3′5′-CATCACGGGACACCCATTC-3′
MMP-25′-CCTGGGCCCCGTCACT-3′5′-GAGATGCCGTCGAAGACGAT-3′
MMP-95′-TTAGGAACCGCTTGCATTTCTT-3′5′-CCCCCTCCCTCAGAAAGTCT-3′
18s rRNA5′-CGAACGTCTGCCCTATCAACTT-3′5′-ACCCGTGGTCACCATGGTA-3′

ANF, atrial natriuretic factor (NM_174124); CTGF, connective tissue growth factor (NM_174030); MMP-2, matrix metalloproteinase-2 (NM_174745); MMP-9, matrix metalloproteinase-9 (NM_174744); 18s rRNA, 18s ribosomal RNA (eukaryotic sequence).