Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification
Figure 3
Establishment of criteria for prognostication of patients with ductal adenocarcinomas of the pancreas. (a) Scattergrams of the signal ratios in samples of cancerous tissue obtained from patients in the no-relapse group (NR, ) and early-relapse group (ER, ) who had not undergone adjuvant chemotherapy with gemcitabine after surgery on representative bacterial artificial chromosome (BAC) clones, RP11-165B11 and RP11-92A14. Using the cutoff values indicated by the dotted lines, patients belonging to the ER-group were discriminated from those belonging to the NR-group in the learning cohort with 100% specificity. (b) Histogram showing the number of BAC clones satisfying the criteria listed in Table 3 for patients belonging to the NR- (shaded column) and ER- (filled columns) groups in the learning cohort. (c) Kaplan-Meier survival curves of 34 patients who had not undergone adjuvant chemotherapy with gemcitabine after surgery in the validation cohort. Both the recurrence-free and overall survival rates of 29 patients satisfying the criteria listed in Table 3 for 2 or more BAC clones (solid lines) were significantly lower than those of 5 patients satisfying the criteria listed in Table 3 for less than 2 BAC clones (dotted lines). Log-rank test ( and , resp.).
