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Figure 10: Soluble heparin blocks HVS-GFP-BAC entry in Huh7.5 cells and inhibits HVS mORF51 to a lesser extent. Cells were incubated with a range of concentrations of heparin in 5% media for 1 h prior to infection with virus (also in 5% media). 48 h after infection, cells were removed from their wells, and GFP fluorescence was measured by flow cytometry ( ). Concentrations of heparin over 0.05 mg/ml resulted in viral cell entry being inhibited with both viruses. However, the neutralisation effect is less severe with HVS mORF51, suggesting some attenuation of its heparin-binding capacity.