Mutation of Herpesvirus Saimiri ORF51 Glycoprotein Specifically Targets Infectivity to Hepatocellular Carcinoma Cell Lines

Figure 5

Schematic describing the resolution of cointegrant clones. Cointegrants which have the pKOV Kan-mORF51 plasmid (shown in blue) inserted into the HVS genome (shown in grey) undergo a second recombination event, resulting in two possible outcomes. If the recombination site is in the same homology region as the first recombination, the intact pKOV Kan mORF51 plasmid is excised, forming a revertant. However, if this recombination occurs in the opposite homology region to the previous recombination event, the mutated region (shown in red) remains in the HVS genome, while the wild-type ORF51 is incorporated into the pKOV Kan plasmid.