Research Article

Influence of Hsp90 and HDAC Inhibition and Tubulin Acetylation on Perinuclear Protein Aggregation in Human Retinal Pigment Epithelial Cells

Figure 1

(a) Phase contrast micrographs of control (C) cells, and cells exposed to 5  M MG-132 (MG) or 0.25  M geldanamycin (GA) or 1  M trichostatin A (TSA) or 1  M taxol (TAX) or 5  M nocodazole (NOC) for 24 hours. In addition, the cells were treated simultaneously with GA or TSA or TAX or NOC and MG-132 for up to 24 hours, (b) and then allowed to recover under the indicated conditions for 24 hours. Protein aggregates are seen as dark granular deposits in perinuclear space (downward arrows), the examples of clear cytoplasm are marked with upward arrows. Arrows from left to right indicate disperse aggregation.
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(a)
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(b)