Influence of Hsp90 and HDAC Inhibition and Tubulin Acetylation on Perinuclear Protein Aggregation in Human Retinal Pigment Epithelial Cells
Figure 4
(a) Total proteins (20 g) of whole cell extracts were examined by western blot using antibodies against LC3 of control (med) cells, and cells exposed 0.25 M geldanamycin (GA) or 5 M MG-132 (MG) or their combination for 6, 12, and 24 hours. Tubulin was used to check the equal loading of proteins. (b) Quantifications of western blots and LC3 II/I ratio.
