Figure 1: Characterization of CAG/hNEDL1 transgenic mice. (a) Schematic representation of CAG/hNEDL1 construction, in which CAG promoter contains the cytomegalovirus enhancer and a chicken -actin promoter and intron. (b) Semiquantitative RT-PCR analyses of the RNA samples extracted from liver, skeletal muscle, kidney, and brain of both wild-type and transgenic mice were carried out and GAPDH was used as control in this test. (c) Western blotting analyses of human hNEDL1 protein in different tissues of both wild-type and hNEDL1-Tg mice were carried out and -actin was used as loading control.