In Vitro Culture Conditions for Maintaining a Complex Population of Human Gastrointestinal Tract Microbiota

Table 1

Medium components for the optimal growth of intestinal microbiota^a.

Chemical component	Conc. (g L^−1)		Chemical component	Conc. (g L^−1)
	High	Low		High	Low
Starch	5.0	0.5	FeSO4⋅7H2O	0.005	0.005
Pectin (citrus)	2.0	0.5	NaCl	4.5	4.5
Guar gum	1.0	0.5	KCl	4.5	4.5
Porcine gastric typeIII mucin	4.0	0.5	KH2PO4	0.5	0.5
Xylan (Oat-spelt)	2.0	0.5	MgSO4⋅7H2O	1.25	1.25
Arabinogalactan (larch wood)	2.0	0.5	CaCl2⋅6H2O	0.15	0.15
Inulin	1.0	1.0	NaHCO3	1.5	1.5
Casein	3.0	3.0	Cysteine	0.8	0.8
Peptone water	5.0	0.5	Hemin	0.05	0.05
Tryptone	5.0	0.5	Tween 80	1.0	1.0
Bile salts no.3	0.4	0.4	Resazurin; (0.01%)	1 mL	1 mL
Yeast extract	4.5	4.5	Vitamin solution	1 mL	1 mL
Vitamin solution (mg L^−1)
Menadione	1.0		Vitamin B12	0.5
Biotin	2.0		Thiamine	4.0
Pantothenate	10.0		ρ-Aminobenzoic acid	5.0
Nicotinamide	5.0

^aMedia were adapted and modified from previous studies [18, 19].