In Vitro Culture Conditions for Maintaining a Complex Population of Human Gastrointestinal Tract Microbiota

Table 4

The summary of pyrosequencing data and statistical calculations of fecal samples obtained from 3 individuals before and after 18 hours of incubation in low-carbohydrate medium.

				Read length (bp)		Phylotype^b
Individual		Total	Analyzed	Mean	Maximum	Observed	Chao1	Shannon	Goods
		reads	reads^a	length	length	OTUs	estimation	index	coverage
A	0 hr	9,903	8,898	386.68	508	615	1,007.04	5.13	0.97
	18 hr	9,545	7,455	380.65	505	1,287	2,647.51	5.66	0.92
B	0 hr	8,986	8,158	379.22	513	631	1,190.03	5.12	0.96
	18 hr	3,908	3,402	384.63	508	590	1,112.43	5.21	0.92
C	0 hr	8,593	7,786	394.68	511	835	1,725.74	5.20	0.95
	18 hr	4,739	4,132	386.46	511	596	1,136.01	4.93	0.93

^aThe analyzed reads are selected by the length over 300 bp, removing chimera sequences and containing ambiguous nucleotide (N) is 0 or 1 in each sequence.
^bThe cutoff value of phylotype are equal to or greater than 97% similarity.