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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 839653, 4 pages
http://dx.doi.org/10.1155/2011/839653
Research Article

Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1-hVEGF165 by Ultrasound-Targeted Microbubble Destruction

1Department of Ultrasound, Second Affiliated Hospital, Zhejiang University College of Medicine, 88 Jiefang Road, Hangzhou 310009, China
2Department of Cardiology, Second Affiliated Hospital, Zhejiang University College of Medicine, 88 Jiefang Road, Hangzhou 310009, China
3Department of Nuclear Medicine, Second Affiliated Hospital, Zhejiang University College of Medicine, 88 Jiefang Road, Hangzhou 310009, China

Received 29 December 2010; Revised 19 March 2011; Accepted 22 March 2011

Academic Editor: Mei Tian

Copyright © 2011 Zhaoxia Pu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ultrasound-targeted microbubble destruction (UTMD) has been proposed as a new technique for organ-specific gene transfer and drug delivery. This study was performed to investigate the effect of UTMD on marrow mesenchymal stem cells (MSCs) transfected with pcDNA3.1-hVEGF165.pcDNA3.1-hVEGF165 were transfected into the third passage of MSCs, with or without UTMD under different ultrasound conditions. Protein expression was quantified by hVEGF165-ELISA kit after transfection for 24, 48, and 72 hours. UTMD-mediated transfection of MSCs yielded a significant protein expression. UTMD of mechanic index (MI) 0.6 for 90 seconds led to the highest level of protein expression.