Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1⁻-hVEGF₁₆₅ by Ultrasound-Targeted Microbubble Destruction

Table 2

Protein expression of VEGF₁₆₅ in mesenchymal stem cells supernatant after transfection under different ultrasound conditions (𝑛=25, ng/mL).

Various ultrasound conditions		Protein expression of VEGF165
ET	MI	24 h	48 h	72 h
	0.6	118.2±0.7	133.1±0.3	112.7±0.8
30 s	1.0	140.5±1.1	142.0±0.5	131.5±0.1
	1.4	136.6±0.7	154.1±1.1	121.8±0.9
	0.6	177.6±1.2	168.8±2.3	159.1±0.8
60 s	1.0	218.6±0.9	269.2±1.2	199.4±2.1
	1.4	254.6±0.7	289.6±3.6	249.1±0.8
	0.6	289.9±1.5 *	319.1±2.1 *	268.7±1.4 *
90 s	1.0	161.2±1.8	186.5±0.8	151.6±1.3
	1.4	160.0±3.5	175.2±1.6	148.2±2.5

* 𝑃<.05, versus groups with ET 30 s and MI 0.6, 1.0, and 1.4, respectively.