Improvement of Capture Compound Mass Spectrometry Technology (CCMS) for the Profiling of Human Kinases by Combination with 2D LC-MS/MS

Table 1

Proteins and kinases identified in the different LC-MS set-ups.

	Total number of identified proteins in capture assays; (with ≥2 peptides)	Kinases identified in capture assays; (with ≥2 peptides)	Number of competed kinases*; (with ≥2 peptides)
1D LC-MS 90 min	386 (158)	23 (10)	20 (8)
1D LC-MS 180 min	617 (265)	39 (19)	31 (16)
2D LC-MS	1274 (489)	91 (48)	66 (32)

*A kinase is considered to be competed if the number of spectral counts in the capture assay divided by number of spectral counts in the competition control is ≥2 or the number of spectral counts in the competition is =0.