Table 1

MonocultureNonscaffolded cocultureOrganotypic coculture

CompositionSimpleModerate, multicellularComplex 3D, multicellular
InteractivityDirect response to stimulusSynergistic/antagonistic/intercellularSynergistic/antagonistic/intercellul-
ar/stratified
In vivo likenessLeast similarModerately similarBest model of in vivo
Technical requirementsSimple culture and media componentsDistinct media needs, no scaffolding requirementsExtensive expertise in media, culture and perfusion
ContaminationLow riskModerate risk with continuous cell linesModerate risk with continuous cell lines
Architecture considerationsMonolayer or suspensionMixed monolayer, suspension, or combinationScaffolds, gels, permeable barriers, media perfusion