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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 857987, 7 pages
http://dx.doi.org/10.1155/2011/857987
Research Article

Testing a Low Molecular Mass Fraction of a Mushroom (Lentinus edodes) Extract Formulated as an Oral Rinse in a Cohort of Volunteers

1Sezione di Microbiologia, Dipartimento di Patologia e Diagnostica, Università di Verona, Strada Le Grazie 8, 37134 Verona, Italy
2Sezione di Chirurgia Maxillo Facciale e di Odontostomatologia, Dipartimento di Chirurgia, Università di Verona, Piazzale A. Scuro 10, 37134 Verona, Italy
3Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK
4Department of Cariology, Institute of Odontology at Sahlgrenska Academy, University of Gothenburg, Box 450, 405 30 Göteborg, Sweden
5DIPTERIS, Università di Genova, Corso Europa 26, 16132 Genova, Italy
6Dipartmento di Chimica Farmaceutica, Università di Pavia, Via Taramelli 12, 27100 Pavia, Italy
7Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands

Received 15 June 2011; Accepted 11 July 2011

Academic Editor: Carla Pruzzo

Copyright © 2011 Caterina Signoretto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Although foods are considered enhancing factors for dental caries and periodontitis, laboratory researches indicate that several foods and beverages contain components endowed with antimicrobial and antiplaque activities. A low molecular mass (LMM) fraction of an aqueous mushroom extract has been found to exert these activities in in vitro experiments against potential oral pathogens. We therefore conducted a clinical trial in which we tested an LMM fraction of shiitake mushroom extract formulated in a mouthrinse in 30 young volunteers, comparing the results with those obtained in two identical cohorts, one of which received water (placebo) and the other Listerine. Plaque index, gingival index and bacterial counts in plaque samples were determined in all volunteers over the 11 days of the clinical trial. Statistically significant differences ( ) were obtained for the plaque index on day 12 in subjects treated with mushroom versus placebo, while for the gingival index significant differences were found for both mushroom versus placebo and mushroom versus Listerine. Decreases in total bacterial counts and in counts of specific oral pathogens were observed for both mushroom extract and Listerine in comparison with placebo. The data suggest that a mushroom extract may prove beneficial in controlling dental caries and/or gingivitis/periodontitis.