Research Article

Identification of Four Potential Epigenetic Modulators from the NCI Structural Diversity Library Using a Cell-Based Assay

Figure 2

Characterization of cell-based LDR assay. (a) Visualization of GFP expression in cells treated with various known epigenetic modulators. TSA, butyrate, apicidin, and depsipeptide are HDAC inhibitors and 5-aza-deoxycytidine is a DNA methyltransferase inhibitor. (b) Cells exhibit dose-dependent GFP expression in response to HDAC inhibitors. (c) FACS analysis reveals an increase in number of cells expressing GFP when treated with butyrate or apicidin, compared to untreated cells.
868095.fig.002a
(a)
868095.fig.002b
(b)
868095.fig.002c
(c)