868095.fig.003a
(a)
868095.fig.003b
(b)
868095.fig.003c
(c)
868095.fig.003d
(d)
Figure 3: GFP expression requires de novo transcription. (a) Cells treated with 200 nM TSA express GFP. (b) When transcription inhibitor actinomycin-D (0.5 μg/mL) is added to LDR cells together with TSA, no GFP expression is observed. The same pattern is seen with 25 nM depsipeptide, with and without actinomycin-D in (c) and (d), respectively.