Efficient Protein Transduction Method Using Cationic Peptides and Lipids
Figure 3
Wr-T/cationic lipid reagents mixture-mediated selective delivery of proteins into living cells. (a) 3′GST and FLAG tag-NLS VENUS proteins were incubated with Wr-T and cationic lipid reagents and then overlaid onto HeLa cells in serum-free medium for 3 h. The cells were then washed and observed at the same exposure time by fluorescence microscopy. (b) The cells treated with Wr-T/BioPORTER mixture were counterstained with Hoescht 33342.
