Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System
Figure 4
(a) Linearized pLEXSY-hyg2-FVII construct with SwaI (Lanes 1, 2), control plasmid (Lane 3). The arrow head shows a 6500-bp fragment containing cDNA sequence encoding human FVII, hygromycin B resistance gene, 5′ and 3′ portion of the small subunit of L. tarentolaer RNA gene (5′ and 3′ ssu) and three UTRs: utr1, utr2, and utr3. (b) Confirmation of FVII expression by transfected Leishmania cells Lanes (1, 2), Control cells (Lanes 3, 4), Control plasmid (Lane 5).
