Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System

Figure 7

Estimation of the yield of functional rFVII (rFVIIa) by one-stage clotting assay (PT-based assay for FVII). Standard curve of different dilutions of unicalibrator was made. Also, clotting times of dilutions of (1/1), (1/2), (1/5), (1/10) of dialyzed rFVII were recorded. The clotting times of dilutions of dialyzed rFVII were 33, 42, 44, and 48 (s), respectively. The black line mark the (1/1) dilution of dialyzed rFVII activity at the point of intersection of the standard curve through this the yield of functional rFVII (rFVIIa) was estimated 9% of Unicalibrator with 100% FVIIa level. The clotting time of the negative control sample (without FVII) was 60 (s).