903097.fig.001a
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903097.fig.001b
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903097.fig.001c
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903097.fig.001d
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903097.fig.001e
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903097.fig.001f
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903097.fig.001g
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Figure 1: Effect of acute high-dose MTX chemotherapy on growth plate structure and cellular changes in young rats. H & E stained section of a normal rat tibial growth plate (a) and an MTX-treated rat growth plate (b). Dashed line represents total heights of growth plates. BrdU labeling showing proliferative chondrocytes in a normal rat (c) and an MTX-treated rat (d), with arrows pointing to proliferating chondrocytes. Normal proliferative/hypertrophic chondrocytes of a normal rat (e) showing no apoptosis; MTX-treated rats with apoptotic chondrocytes in lower proliferative/upper hypertrophic zone (f), and a magnified view of apoptotic chondrocyte (g). (Images are from the authors’ own lab and have not been published previously.)