903097.fig.003a
(a)
903097.fig.003b
(b)
903097.fig.003c
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903097.fig.003d
(d)
Figure 3: Effect of MTX chemotherapy on osteoclast density in young rats. H & E stained sections showing osteoclasts along trabecular bone surface in a control rat (a) and an MTX-treated rat (b), with arrows pointing to multinucleated osteoclasts. TRAP-stained osteoclasts formed ex vivo from bone marrow cells of a control rat (c) and an MTX-treated rat (d), with arrows pointing to multinucleated TRAP+ osteoclasts. (Images are from the authors’ own lab and have not been published previously.)