Methotrexate Toxicity in Growing Long Bones of Young Rats: A Model for Studying Cancer Chemotherapy-Induced Bone Growth Defects in Children
Figure 3
Effect of MTX chemotherapy on osteoclast density in young rats. H & E stained sections showing osteoclasts along trabecular bone surface in a control rat (a) and an MTX-treated rat (b), with arrows pointing to multinucleated osteoclasts. TRAP-stained osteoclasts formed ex vivo from bone marrow cells of a control rat (c) and an MTX-treated rat (d), with arrows pointing to multinucleated TRAP+ osteoclasts. (Images are from the authors’ own lab and have not been published previously.)
