Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform

Figure 1

Domain structure of utrophin (rat) and dystrophin (human). Numbers denote amino acid (aa) residues according to rat utrophin. The short N-terminal utrophin isoform (N-utro) extends from aa 1 to 539. The black sections UT11, UT12, UT21, UT31, and DYS12 were used for heterologous expression in bacteria and eukaryotic cells. The recombinant proteins were used for production of antibodies, for in vitro actin-binding studies, and for eukaryotic cell transfection. AB, actin-binding domain; H1–H5, hinge regions; R1–R24, spectrin-like triple helical repeats; CR, cystein-rich domain; CT, C-terminal domain.