Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform

Figure 7

Effects of calmodulin and calcium on binding of UT11 and UT12 to actin. Calmodulin plus EGTA does not inhibit actin binding (open symbols). Calmodulin plus Ca²⁺ (black symbols) does not inhibit actin binding of UT11 (a), but binding of UT12 is inhibited (b). Incubation conditions: (O) Ca²⁺ 1 mM; (∆) calmodulin 0.2 mM plus EGTA 1 mM; (●) calmodulin 0.2 mM plus Ca²⁺ 1 mM; (▲) calmodulin 0.2 mM, Ca²⁺ 2 mM plus EGTA 1 mM (for further explanation see the text).

(a)

(b)