Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform

Table 1

Primer used for PCR (for localisation of primers along the cDNA, see Figure 2(a)).

Forward primer	Backward primer
A Rut13F	B Rut23B
C Rut14F	D Rut24B
E Rut18F	F Rut25B
	G Rut28B
H Rut19F	J Rut41B
PCR product size
A-B: 110 bp
A–D: 215 bp
A–F: 326 bp
A–G: 499 bp
C-D: 96 bp
C–F: 207 bp
C–G: 380 bp
E-F: 103 bp
E–G: 276 bp
H-J: 104 bp
Primer sequnce
Forward primer
RUT 13F	5′ CAA AGT GAC CTC GAA GCT GAG C 3′ rat utrophin 1587→1608
RUT 14F	5′ GGG TGA GCG CTG GAC AGC TG 3′ rat utrophin 1706→1725
RUT 18F	5′ GTG CAA CGA CCA TCC ATC AAG G 3′ rat N-utrophin 1810→1831
RUT 19F	5′ GTC TTT TGG AAG CTT GGC TCA CC 3′ rat utrophin 1804→1826
Backward primer
RUT 23B	5′ AAC TGA TCT TCC AAA ACA GCT GTG 3′ rat utrophin 1673⟵1696
RUT 24B	5′ TGT TCT TCC AAT AAT TCC TGC CAC 3′ rat N-utrophin 1778⟵1801
RUT 25B	5′ TTC CAG AGC TCT CAC TAC AGA AG 3′ rat N-utrophin 1890⟵1912
RUT 28B	5′ CCC CAG AAT TAC GAA GGT CAC AC 3′ rat N-utrophin 2063⟵2085
RUT 41B	5′ CAG ACG CCG GAC ACT GAC ACC 3′ rat utrophin 1887⟵1907