Research Article
Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform
Table 1
Primer used for PCR (for localisation of primers along the cDNA, see Figure
2(a)).
| Forward primer | Backward primer |
| A Rut13F | B Rut23B | C Rut14F | D Rut24B | E Rut18F | F Rut25B | | G Rut28B | H Rut19F | J Rut41B |
| PCR product size | |
| A-B: 110 bp | | A–D: 215 bp | | A–F: 326 bp | | A–G: 499 bp | | C-D: 96 bp | | C–F: 207 bp | | C–G: 380 bp | | E-F: 103 bp | | E–G: 276 bp | | H-J: 104 bp | |
| Primer sequnce | |
| Forward primer | |
| RUT 13F | 5′ CAA AGT GAC CTC GAA GCT GAG C 3′ rat utrophin 1587→1608 | RUT 14F | 5′ GGG TGA GCG CTG GAC AGC TG 3′ rat utrophin 1706→1725 | RUT 18F | 5′ GTG CAA CGA CCA TCC ATC AAG G 3′ rat N-utrophin 1810→1831 | RUT 19F | 5′ GTC TTT TGG AAG CTT GGC TCA CC 3′ rat utrophin 1804→1826 |
| Backward primer | |
| RUT 23B | 5′ AAC TGA TCT TCC AAA ACA GCT GTG 3′ rat utrophin 1673⟵1696 | RUT 24B | 5′ TGT TCT TCC AAT AAT TCC TGC CAC 3′ rat N-utrophin 1778⟵1801 | RUT 25B | 5′ TTC CAG AGC TCT CAC TAC AGA AG 3′ rat N-utrophin 1890⟵1912 | RUT 28B | 5′ CCC CAG AAT TAC GAA GGT CAC AC 3′ rat N-utrophin 2063⟵2085 | RUT 41B | 5′ CAG ACG CCG GAC ACT GAC ACC 3′ rat utrophin 1887⟵1907 |
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