Tissue Expression and Actin Binding of a Novel N-Terminal Utrophin Isoform

Table 3

Actin binding of recombinant protein fragments of utrophin and dystrophin. Parameters are derived from the binding data given in Figure 6.

Recombinant protein	Amino acids	Affinity constants (M^−1)		Binding sites per actin monomer		Correlation coefficient (𝑟)	Total actin monomers per ligand at high affinity	Total actin monomer per ligand at saturation
		𝐾1	𝐾2	𝑛1	𝑛2
UT11	2–594	6.3 × 10^6	9.3 × 10^4	0.04	0.28	0.93	25	3.1
UT12	2–261	4.6 × 10^6	2.5 × 10^5	0.14	0.62	0.98	7	1.3
DYS12	1–246	1.3 × 10^5	3.7 × 10^5	1.90 (𝑛1 plus 𝑛2)		0.98	—	1.9