Quantitative Proteomics Analysis by Isobaric Tags for Relative and Absolute Quantitation Identified Lumican as a Potential Marker for Acute Aortic Dissection

Table 2

List of the increased (>2-fold) protein targets identified and their corresponding class, associated biological process, and cellular component.

N	Unused^a	Peptides^b	Accession #	Name	Biological process	Cellular component	Protein class	AAD/CON ratio	AAD/AMI ratio
1	2.7	1	Q13509	Tubulin beta-3 chain	Cellular component morphogenesis	Cytoskelelton	Cytoskeletal protein/tubulin	39.8406	0.0711
2	2	1	P01600	Ig kappa chain V-I region Hau			Unclassified	31.3480	3.0760
3	6.67	3	P02743	Serum amyloid P-component	Response to stress		Defense/immunity protein/antibacterial response	24.4499	9.1241
4	13.53	10	P05546	Heparin cofactor 2	Protein metabolic process		Enzyme modulator	19.7628	11.2740
5	17.67	9	P36955	Pigment epithelium-derived factor	Protein metabolic process		Enzyme modulator	19.7628	9.8135
6	6.24	4	P05543	Thyroxine-binding globulin	Protein metabolic process		Enzyme modulator	12.7065	3.7665
7	12.63	18	P01834	Ig kappa chain C region	Response to stimulus	Immunoglobulin complex	Defense/immunity protein	11.6959	1.8031
8	55.14	43	P02751	Fibronectin	Blood coagulation	Extracellular matrix	Transfer/carrier protein	11.5875	3.7327
9	35.94	29	P01011	Alpha-1-antichymotrypsin	Protein metabolic process		Enzyme modulator	10.5708	8.0906
10	2.22	1	P02741	C-reactive protein	Response to stress		Defense/immunity protein	9.1241	5.9701
11	2	1	Q9UK55	Protein Z-dependent protease inhibitor	Protein metabolic process		Enzyme modulator	8.7873	2.4888
12	100.78	51	P04114	Apolipoprotein B-100	Lipid metabolic process		Transfer/carrier protein	8.6281	2.8050
13	9.19	4	P35858	Insulin-like growth factor-binding protein complex acid labile chain	Cell-cell adhesion	Extracellular matrix	Receptor	8.4746	4.0177
14	47.88	42	P19827	Inter-alpha-trypsin inhibitor heavy chain H1	Protein metabolic process		Enzyme modulator	5.8072	5.6497
15	106.72	89	P00450	Ceruloplasmin	Blood coagulation	Extracellular matrix	Transporter	5.1046	9.6339
16	37.56	23	P04196	Histidine-rich glycoprotein	Blood coagulation		Unclassified	4.6125	3.2206
17	4.15	2	P08571	Monocyte differentiation antigen CD14	Immune system process		Receptor	4.0933	2.4888
18	2.72	1	Q96KN2	Beta-Ala-His dipeptidase	Protein metabolic process		Protease	4.0933	1.9231
19	31.66	20	P01871	Ig mu chain C region	Response to stimulus		Defense/immunity protein	3.8023	2.3552
20	12.12	6	P51884	Lumican	Cell-cell adhesion	Extracellular matrix	Receptor	3.6311	1.2942
21	2.71	1	P00740	Coagulation factor IX	Blood coagulation		Protease	3.4037	2.0137
22	6.27	3	P08185	Corticosteroid-binding globulin	Protein metabolic process		Enzyme modulator	3.2808	5.4945
23	2.58	21	P00739	Haptoglobin-related protein			Unclassified	3.2206	1.7538
24	4.98	4	P04003	C4b-binding protein alpha chain	Blood coagulation		Transfer/carrier protein	3.1626	5.0582
25	6.02	6	P02745	Complement C1q subcomponent subunit A	Response to stimulus		Transfer/carrier protein	3.1046	3.6982
26	5.83	3	P22792	Carboxypeptidase N subunit 2	Cell adhesion	Extracellular matrix	Receptor	3.0202	1.3805
27	15.99	11	P05155	Plasma protease C1 inhibitor	Protein metabolic process		Enzyme modulator	2.9647	1.2824
28	2.02	2	P01742	Ig heavy chain V-I region EU			Unclassified	2.8580	1.1695
29	2	1	P09486	SPARC	Cell-cell signaling		Transfer/carrier protein	2.7042	0.7312
30	18.06	9	P02760	Protein AMBP	Blood coagulation		Enzyme modulator	2.6062	3.5651
31	8.99	5	P12259	Coagulation factor V	Blood coagulation	Extracellular matrix	Transporter	2.6062	3.2206
32	8.31	6	P27169	Serum paraoxonase/arylesterase 1	Immune system process		Oxidoreductase	2.5349	5.6497
33	2.32	1	P19320	Vascular cell adhesion protein 1	Cell-cell adhesion		Defense/immunity protein	2.3121	2.1478
34	2.01	1	O00187	Mannan-binding lectin serine protease 2	Response to stimulus		Protease	2.3121	1.5277
35	43.19	29	P02749	Beta-2-glycoprotein 1	Blood coagulation		Transfer/carrier protein	2.2287	7.1124
36	3.52	2	P11226	Mannose-binding protein C	Response to stimulus		Defense/immunity protein	2.2080	2.6062
37	87.93	67	P00738	Haptoglobin	Blood coagulation		Protease	2.1678	3.5651
38	5.7	4	Q13790	Apolipoprotein F	Lipid metabolic process		Transporter	2.1478	3.9448
39	65.42	41	P02790	Hemopexin	Vitamin transport		Transfer/carrier protein	2.1478	3.9078
40	2.96	1	P20851	C4b-binding protein beta chain	Blood coagulation		Transfer/carrier protein	2.1281	4.8309
41	2	1	P61769	Beta-2-microglobulin	Response to stimulus		Defense/immunity protein	2.1088	2.1281
42	20.86	10	P02748	Complement component C9	Response to stimulus		Receptor	2.1088	0.6252
43	6.96	3	P07477	Trypsin-1	Protein metabolic process		Protease	2.1088	0.3436
44	13.64	7	P05156	Complement factor I	Response to stimulus		Protease	2.0325	0.9120
45	15.17	10	P01842	Ig lambda chain C regions	Response to stimulus	Immunoglobulin complex	Defense/immunity protein	2.0137	2.1678
46	4.29	1	P35030	Trypsin-3	Protein metabolic process		Protease	2.0137	1.3931

^a Unused > 1.3 means at least 95% confidence; ^bnumber of peptides with 95% confidence; AAD: acute aortic dissection; AMI: acute myocardial infarction; CON: normal controls.