BAC Modification through Serial or Simultaneous Use of CRE/Lox Technology
Figure 2
Comparison of reaction rates of Lox variant sites. (a) Fragment bearing Lox sites used as reactant on top with products resulting from Cre-mediated excision of reactant below. Shown on the bottom is gel electrophoresis to separate Cre-mediated reaction fragments. Time in minutes indicated at top of each lane. Identity of Lox variant is denoted below each time course. Identity of bands denoted at right of gel. (b)–(d) Reaction progress plotted as the ratio of products/reactants +/− SEM. (b) LoxP in magenta, Lox5171 in orange, Lox2722 in blue, and Loxm2 in purple. (c) LR-LoxP (LoxP-66-LoxP-71) pair in maroon, LR-Lox5171 in teal, LR-Lox2722 in blue, and LR-Loxm2 in light blue. (d) Average of all Lox spacer variants with (orange) or without (blue) inverted repeat variants.
