Comparison of Myocardial Remodeling between Cryoinfarction and Reperfused Infarction in Mice
Figure 1
Comparison of myocardial histopathology between cryoinfarction and reperfused infarction. Hematoxylin-eosin staining of left ventricular lateral wall in cryoinfarction after (a) 3 days an increased cellular infiltration with extensive cardiomyocyte debris, with (b) formation of granulation tissue after 7 days, and (c) an almost compacted scar formation after 14 days postinjury with permanent occlusion of a large coronary vessel (arrow). In parts (a)-(c), a higher magnification (400x) insert shows cellular and matrix content in detail. In contrast, (d) reperfused myocardial infarction shows granulation tissue formation after 3 days and (e) a compacted, nontransmural scar formation after 7 days reperfusion. Picrosirius red staining of (f) cryoinfarction after 7 days reveals loose collagen fibers, while (g) reperfused infarction presents a compacted, collagen-rich scar. Alpha smooth muscle actin staining of myofibroblasts (black-stained cells) indicates (h) active interstitial remodeling 7 days after cryoinfarction whereas (i) reperfused infarction has only few positive cells in the scar (eosin counterstaining). Scale bar: 150 μm.
