Research Article
Identification of Urinary Biomarkers of Colon Inflammation in IL10-/- Mice Using Short-Column LCMS Metabolomics
Table 1
Biomarker Candidate ions (m/z) selected from Short-Column LCMS and LC-MS/MS analysis of urine samples from IL10-/- and wild-type (WT) mice.
| | Short-Column LCMS Biomarker ion () | Average fold change Log2 (IL10−/−/WT) | value | Early difference observed | Identification, |
| | Negative ions | | | | |
| | 204 | 1.8 (expt. 1) | .01 (FDR 0.05) | Y | xanthurenic acid | | 2.5 (expt. 2) | .01 (FDR 0.05) | | | | 308 | −1.5 (expt. 1) | .01 (FDR 0.05)) | ? (expt. 1) | unknown | | −1.8 (expt. 2) | .01 (FDR 0.05) | Y (expt. 2) | | | 380 | −1.1 (expt. 1) | .01 | N (expt. 1) | xanthurenic acid glucuronide | | −1.6 (expt. 2) | .01 | Y (expt. 2) | | | 453 | −1.5 (expt. 1) | .01 (FDR 0.05) | N (expt. 1) | -CEHC glucuronide | | −2.0 (expt. 2) | .01 (FDR 0.05) | Y (expt. 2) | | | 495 | −1.5 (expt. 1) | .01 (FDR 0.05) | Y (expt. 1) | unknown | | −2.2 (expt. 2) | .01 (FDR 0.05) | Y (expt. 2) | |
| | Positive ions | | | | |
| | 206 | 1.7 (expt. 1) | .01 (FDR 0.05) | Y (expt. 1) | xanthurenic acid | | 1.2 (expt. 2) | .01 | N (expt. 2) | | | 382 | −1.2 (expt. 1) | .01 | N (expt. 1) | xanthurenic acid glucuronide | | −1.5 (expt. 2) | .01 (FDR 0.05) | Y (expt. 2) | | | 497 | −1.2 (expt. 1) | .01 | Y (expt. 1) | unknown | | −1.4 (expt. 2) | .01 (FDR 0.05) | Y (expt. 2) | |
|
|
