Novel Image Analysis Approach Quantifies Morphological Characteristics of 3D Breast Culture Acini with Varying Metastatic Potentials
Figure 3
MCF10 series cell lines exhibit variation in acinar structure morphology along the metastatic cascade after 14 days in 3D laminin rich environment culture systems. Cells were cultured in 3D Matrigel suspensions and stained with integrin 3 (red), integrin 6 (green), and DAPI (blue). Example image slices from the six cell lines are typical of those captured during the course of the experiments and are displayed from (a) to (f) as follows: 10A, AT, KCL, DCIS, CA1H, and CA1A.
